This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A new analytical method for d-alanine (d-Ala) based on capillary electrophoretic separation and optical fiber light-emitting diode (LED)-induced fluorescence detection has been developed. Fluorescein isothiocyanate (FITC) was used for precolumn derivatization of amino acids. The separation and determination of the derivatives was performed using a novel laboratory-built CE system with optical fiber LED-induced fluorescence detector. Optimal separation was performed at 15kV using a background electrolyte solution consisted of 25mmolL^-^1 sodium borate (pH 9.5), 18mmolL^-^1 @b-CD and 15mmolL^-^1 SDS. High sensitive detection was obtained by the optical fiber LED-induced fluorescence detection using a blue LED as the excitation source. The limits of detection (S/N=3) for d-Ala was 8.0×10^-^9molL^-^1. A calibration curve ranging from 2.0×10^-^8molL^-^1 to 1.0×10^-^6molL^-^1 was shown to be linear. Using this method, the levels of d-Ala in human plasma from healthy donors were determined.
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